Flow injection and HPLC determination of furosemide using pulsed amperometric detection at microelectrodes

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 33; no. 5; pp. 923 - 933
• Main Authors: Guzmán, Alicia, Agüı́, Lourdes, Pedrero, Marı́a, Yáñez-Sedeño, Paloma, Pingarrón, José M
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 04.12.2003; Elsevier Science
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Electrochemistry; Flow Injection Analysis - methods; Fundamental and applied biological sciences. Psychology; Furosemide; Furosemide - analysis; Furosemide - chemistry; General pharmacology; Medical sciences; Microelectrodes; Milk; Pharmacology. Drug treatments; Pulsed amperometric detection; Pulsed amperometric detection; Microelectrodes; Furosemide; Milk; Sulfanilamide derivatives; Sulfonamide; HPLC chromatography; Diuretic; Anthranilic acid derivatives; Antihypertensive agent; Detection; Quantitative analysis; Flow injection

The flow-injection and HPLC determination of the diuretic drug furosemide using pulsed amperometric detection (PAD) at cylindrical carbon fibre microelectrodes (CFMEs) is reported. Experimental conditions such as pH (6.5) and buffer concentration (0.05 mol l −1 HPO 4 2−/H 2PO 4 −) were optimized usi...