Ca(2+)-independent cytosolic phospholipase A2 from macrophage-like P388D1 cells. Isolation and characterization

• Published in: The Journal of biological chemistry Vol. 269; no. 12; pp. 9227 - 9233
• Main Authors: Ackermann, E J, Kempner, E S, Dennis, E A
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 25.03.1994
• Subjects: Adenosine Triphosphate - chemistry; Animals; Calcium - metabolism; Cytosol - enzymology; Enzyme Activation; Leukemia P388 - enzymology; Macrophages - enzymology; Microsomes - enzymology; Molecular Weight; Octoxynol - chemistry; Phospholipases A - isolation & purification; Phospholipases A - metabolism; Phospholipases A2; Substrate Specificity

A novel form of an ATP-regulated, oligomeric, Ca(2+)-independent phospholipase A2 (iPLA2) has been purified from the cytosol of the murine macrophage-like cell line P388D1. The purification procedure included ammonium sulfate precipitation and sequential column chromatography on octyl-Sepharose, ATP...