Antibodies to biotinylated red blood cells in adults and infants: improved detection, partial characterization, and dependence on red blood cell‐biotin dose

• Published in: Transfusion (Philadelphia, Pa.) Vol. 57; no. 6; pp. 1488 - 1496
• Main Authors: Schmidt, Robert L., Mock, Donald M., Franco, Robert S., Cohen, Robert M., North, Anne K., Cancelas, José A., Geisen, Christof, Strauss, Ronald G., Vlaar, Alexander P., Nalbant, Demet, Widness, John A.
• Format: Journal Article
• Language: English
• Published: United States 01.06.2017
• Subjects: Adult; Agglutination Tests; Antibodies - immunology; Biological Assay - methods; Biotin - chemistry; Biotinylation; Erythrocytes - immunology; Female; Humans; Infant; Infant, Newborn; Male; Succinimides - chemistry
• ISSN: 0041-1132; 1537-2995; 1537-2995
• DOI: 10.1111/trf.14075

BACKGROUND Biotin‐labeled red blood cells (BioRBCs) are used for in vivo kinetic studies. Because BioRBC dosing occasionally induces antibodies, a sensitive and specific anti‐BioRBC detection assay is needed. STUDY DESIGN AND METHODS Aims were to 1) develop a gel card assay to evaluate existing, nat...