Disruption and overexpression of 6-phosphofructo-2-kinase influence organic acid production in Aspergillus carbonarius ITEM 5010

• Published in: World journal of microbiology & biotechnology Vol. 36; no. 7; p. 98
• Main Authors: Yang, Lei, Nilsson, Lena, Lübeck, Mette, Ahring, Birgitte K., Bruno, Kenneth S., Lübeck, Peter S.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.07.2020; Springer Nature B.V
• Subjects: 6-Phosphofructo-2-kinase; Acid production; Acids; Acids - metabolism; Allosteric properties; Applied Microbiology; Aspergillus - enzymology; Aspergillus - genetics; Aspergillus - metabolism; Aspergillus carbonarius; Biochemistry; Biomedical and Life Sciences; Biotechnology; Citric acid; Citric Acid - metabolism; Disruption; Environmental Engineering/Biotechnology; Gene Expression Regulation, Fungal; Gene Knockout Techniques; genes; Genes, Fungal - genetics; Glycolysis; Kinases; Life Sciences; Malates - metabolism; Malic acid; Microbiology; Organic acids; Organic Chemicals - metabolism; Original Paper; Phosphofructokinase-2 - genetics; Phosphofructokinase-2 - metabolism; sugars; Transcriptome; 6-Phosphofructo-2-kinase; Citric acid; Organic acids; Aspergillus carbonarius

Aspergillus carbonarius is an efficient producer of organic acids with great potential for bio-based production of organic acids. In this study, we identified a gene f2kp encoding the enzyme 6-phosphofructo-2-kinase known as an allosteric regulator of the glycolytic flux and investigated its role in...