Ultra-thin membrane filter with a uniformly arrayed nanopore structure for nanoscale separation of extracellular vesicles without cake formation
Extracellular vesicles (EVs) have emerged as vehicles that mediate diverse cell-cell communication. However, in-depth understanding of these vesicles is hampered by a lack of a reliable isolation method to separate different types of EVs with high levels of integrity and purity. Here, we developed a...
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Published in | Nanoscale advances Vol. 5; no. 3; pp. 64 - 649 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
RSC
31.01.2023
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Subjects | |
Online Access | Get full text |
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Summary: | Extracellular vesicles (EVs) have emerged as vehicles that mediate diverse cell-cell communication. However, in-depth understanding of these vesicles is hampered by a lack of a reliable isolation method to separate different types of EVs with high levels of integrity and purity. Here, we developed a nanoporous and ultra-thin membrane structure (NUTS) that warrants the size-based isolation of EVs without cake formation, minimizing the sample loss during the filtration process. By utilizing the micro-electro-mechanical systems (MEMS) technique, we could also control the pore size in nanoscale. We validated the performance of this membrane to separate EVs according to their size range.
We achieved filtration of an extracellular vesicle (EV) enrichment sample using a nanoporous and ultra-thin membrane structure (NUTS). It allows precise control of the pore size of the membrane
via
MEMS, enabling accurate size-based isolation. |
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Bibliography: | Electronic supplementary information (ESI) available: SEM images of a commercial filter membrane, schematic diagram of fabrication of NMUS, relationships of pressure with various factors, optical image of a NMUS surface, distribution of pore diameter in single membrane, roughness of NMUS https://doi.org/10.1039/d2na00227b AFM and partial fraction of NMUS by AFM indentation, SEM image of the membrane after bio-sample filtration, blocking analysis of bio-sample filtration, western blot data for the apoptotic body markers, contact angle measurement of the membrane surface, design of fluidic channel, microarray images of Exoview measurement. See DOI via ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2516-0230 2516-0230 |
DOI: | 10.1039/d2na00227b |