Development of a tick-borne pathogen QPCR panel for detection of Anaplasma, Ehrlichia, Rickettsia, and Lyme disease Borrelia in animals

• Published in: Journal of microbiological methods Vol. 151; pp. 83 - 89
• Main Authors: Shen, Zhenyu, Zhang, Michael Z., Stich, Roger W., Mitchell, William J., Zhang, Shuping
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2018
• Subjects: Anaplasma; Anaplasma - genetics; Anaplasma - isolation & purification; Anaplasma - pathogenicity; Animals; Borrelia; Borrelia - genetics; Borrelia - isolation & purification; Borrelia - pathogenicity; Ehrlichia; Ehrlichia - genetics; Ehrlichia - isolation & purification; Ehrlichia - pathogenicity; Horse Diseases - microbiology; Horses; Lyme Disease - diagnosis; Lyme Disease - microbiology; Molecular Diagnostic Techniques - methods; Real-time PCR; Real-Time Polymerase Chain Reaction - methods; Rickettsia; Rickettsia - genetics; Rickettsia - isolation & purification; Rickettsia - pathogenicity; Sensitivity and Specificity; Temperature; Tick-borne; Tick-Borne Diseases - diagnosis; Tick-Borne Diseases - microbiology; Tick-Borne Diseases - veterinary; Ticks - microbiology; Tick-borne; Anaplasma; Borrelia; Real-time PCR; Rickettsia; Ehrlichia

Anaplasma spp., Ehrlichia spp., Rickettsia spp., and Lyme disease associated Borrelia spp. are the most common tick-borne pathogens reported to infect human beings worldwide and other animals, such as dogs and horses. In the present study, we developed a broad-coverage SYBR Green QPCR panel consisti...