Proteomic profiling of nipple aspirate fluid (NAF): Exploring the complementarity of different peptide fractionation strategies

• Published in: Journal of proteomics Vol. 117; pp. 86 - 94
• Main Authors: Brunoro, Giselle Villa Flor, Carvalho, Paulo Costa, Ferreira, André Teixeira da Silva, Perales, Jonas, Valente, Richard Hemmi, de Moura Gallo, Claudia Vitória, Pagnoncelli, Dante, Neves-Ferreira, Ana Gisele da Costa
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 18.03.2015
• Subjects: Adult; Breast fluid; Female; Fibroadenoma - metabolism; Humans; Mass Spectrometry; NAF; Neoplasm Proteins - metabolism; Nipple Aspirate Fluid - metabolism; Peptides - metabolism; Pre-fractionation; Proteomics; Shotgun; Tumor Microenvironment; Pre-fractionation; Shotgun; NAF; Breast fluid

NAF is a breast fluid that is closely related to the tumor microenvironment and a valuable sample for studying breast cancer. To perform an in-depth proteomic analysis of this sample, aliquots of a single NAF digest were analyzed by the following peptide-centric fractionation strategies: a) 30-cm re...