An extended gate FET-based biosensor integrated with a Si microfluidic channel for detection of protein complexes
In this work, we present an extended gate field effect transistor (EGFET)-based biosensor integrated with a silicon micro-fluidic channel for the electronic detection of streptavidin–biotin protein complexes. The connection between the EGFET and microfluidic system could be achieved with the propose...
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| Published in | Sensors and actuators. B, Chemical Vol. 117; no. 2; pp. 488 - 494 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
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Elsevier B.V
12.10.2006
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| Subjects | |
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| Abstract | In this work, we present an extended gate field effect transistor (EGFET)-based biosensor integrated with a silicon micro-fluidic channel for the electronic detection of streptavidin–biotin protein complexes. The connection between the EGFET and microfluidic system could be achieved with the proposed device, as it offers isolation between the device and solution, compatibility with the integrated circuit (IC) technology and, is applicable to the micro total analysis system (μ-TAS). The device was fabricated on the basis of semiconductor IC fabrication and micro-electro mechanical system (MEMS) technology. Au was used as the extended gate metal to form a self-assembled monolayer (SAM) with thiol. The bindings of the SAM, streptavidin and biotin were detected by measuring the electrical characteristics of the FET device. We also verified the interactions among the SAM, streptavidin, and biotin by using surface plasmon resonance (SPR) measurements. Furthermore, atomic force microscopy (AFM) images of the bio-layers formed on the Au electrode were taken in a solution in order to determine the presence of protein biomolecules with the proposed configuration. |
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| AbstractList | In this work, we present an extended gate field effect transistor (EGFET)- based biosensor integrated with a silicon micro-fluidic channel for the electronic detection of streptavidin-biotin protein complexes. The connection between the EGFET and microfluidic system could be achieved with the proposed device, as it offers isolation between the device and solution, compatibility with the integrated circuit (IC) technology and, is applicable to the micro total analysis system ( mu -TAS). The device was fabricated on the basis of semiconductor IC fabrication and micro-electro mechanical system (MEMS) technology. Au was used as the extended gate metal to form a self-assembled monolayer (SAM) with thiol. The bindings of the SAM, streptavidin and biotin were detected by measuring the electrical characteristics of the FET device. We also verified the interactions among the SAM, streptavidin, and biotin by using surface plasmon resonance (SPR) measurements. Furthermore, atomic force microscopy (AFM) images of the bio-layers formed on the Au electrode were taken in a solution in order to determine the presence of protein biomolecules with the proposed configuration. In this work, we present an extended gate field effect transistor (EGFET)-based biosensor integrated with a silicon micro-fluidic channel for the electronic detection of streptavidin–biotin protein complexes. The connection between the EGFET and microfluidic system could be achieved with the proposed device, as it offers isolation between the device and solution, compatibility with the integrated circuit (IC) technology and, is applicable to the micro total analysis system (μ-TAS). The device was fabricated on the basis of semiconductor IC fabrication and micro-electro mechanical system (MEMS) technology. Au was used as the extended gate metal to form a self-assembled monolayer (SAM) with thiol. The bindings of the SAM, streptavidin and biotin were detected by measuring the electrical characteristics of the FET device. We also verified the interactions among the SAM, streptavidin, and biotin by using surface plasmon resonance (SPR) measurements. Furthermore, atomic force microscopy (AFM) images of the bio-layers formed on the Au electrode were taken in a solution in order to determine the presence of protein biomolecules with the proposed configuration. In this work, we present an extended gate field effect transistor (EGFET)-based biosensor integrated with a silicon micro-fluidic channel for the electronic detection of streptavidin-biotin protein complexes. The connection between the EGFET and microfluidic system could be achieved with the proposed device, as it offers isolation between the device and solution, compatibility with the integrated circuit (IC) technology and, is applicable to the micro total analysis system (D*D*m-TAS). The device was fabricated on the basis of semiconductor IC fabrication and micro-electro mechanical system (MEMS) technology. Au was used as the extended gate metal to form a self-assembled monolayer (SAM) with thiol. The bindings of the SAM, streptavidin and biotin were detected by measuring the electrical characteristics of the FET device. We also verified the interactions among the SAM, streptavidin, and biotin by using surface plasmon resonance (SPR) measurements. Furthermore, atomic force microscopy (AFM) images of the bio-layers formed on the Au electrode were taken in a solution in order to determine the presence of protein biomolecules with the proposed configuration. |
| Author | Shin, Jang-Kyoo Kim, Dong-Sun Kim, Pan Kyeom Park, Jee-Eun Lim, Geunbae Shoji, Shuichi |
| Author_xml | – sequence: 1 givenname: Dong-Sun surname: Kim fullname: Kim, Dong-Sun organization: School of Electronic and Electrical Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea – sequence: 2 givenname: Jee-Eun surname: Park fullname: Park, Jee-Eun organization: Department of Sensor Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea – sequence: 3 givenname: Jang-Kyoo surname: Shin fullname: Shin, Jang-Kyoo email: jkshin@ee.knu.ac.kr organization: School of Electronic and Electrical Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea – sequence: 4 givenname: Pan Kyeom surname: Kim fullname: Kim, Pan Kyeom organization: Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea – sequence: 5 givenname: Geunbae surname: Lim fullname: Lim, Geunbae organization: Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea – sequence: 6 givenname: Shuichi surname: Shoji fullname: Shoji, Shuichi organization: Department of Electrical Engineering and Bioscience Engineering, Waseda University, Tokyo, Japan |
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| Keywords | Bio-AFM Microfluidic channel Protein sensor SPR EGFET FET-type biosensor |
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| Snippet | In this work, we present an extended gate field effect transistor (EGFET)-based biosensor integrated with a silicon micro-fluidic channel for the electronic... In this work, we present an extended gate field effect transistor (EGFET)- based biosensor integrated with a silicon micro-fluidic channel for the electronic... |
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| SubjectTerms | Bio-AFM EGFET FET-type biosensor Microfluidic channel Protein sensor SPR |
| Title | An extended gate FET-based biosensor integrated with a Si microfluidic channel for detection of protein complexes |
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