Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Human Immature Dental Pulp Stem Cells

• Published in: Investigative ophthalmology & visual science Vol. 51; no. 3; p. 1408
• Main Authors: Gomes, Jose Alvaro Pereira, Geraldes Monteiro, Babyla, Melo, Gustavo Barreto, Smith, Ricardo Luiz, Cavenaghi Pereira da Silva, Marcelo, Lizier, Nelson Foresto, Kerkis, Alexandre, Cerruti, Humberto, Kerkis, Irina
• Format: Journal Article
• Language: English
• Published: United States ARVO 01.03.2010
• Subjects: Animals; Burns, Chemical - pathology; Burns, Chemical - surgery; Cell Culture Techniques; Corneal Diseases - surgery; Dental Pulp - cytology; Disease Models, Animal; Eye Burns - chemically induced; Eye Burns - pathology; Eye Burns - surgery; Fluorescent Antibody Technique, Indirect; Humans; Limbus Corneae - surgery; Limbus Corneae - ultrastructure; Mesenchymal Stem Cell Transplantation; Mesenchymal Stromal Cells - cytology; Microscopy, Electron, Transmission; Ophthalmologic Surgical Procedures; Rabbits; Reconstructive Surgical Procedures; Tissue Engineering; Transplantation, Heterologous

To determine the outcome of the use of a tissue-engineered cell sheet composed of human undifferentiated immature dental pulp stem cells (hIDPSC) for ocular surface reconstruction in an animal model of total limbal stem cell deficiency (LSCD). LSCD was induced by the application of 0.5 M NaOH to the...