Continuous alkaline pretreatment of Miscanthus sacchariflorus using a bench-scale single screw reactor
Miscanthus sacchariflorus 'Goedae-Uksae 1' (GU) was developed as an energy crop of high productivity in Korea. For the practical use of GU for bioethanol production, a bench-scale continuous pretreatment system was developed. The reactor performed screw extrusion, soaking and thermochemica...
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Published in | Bioresource technology Vol. 181; pp. 338 - 344 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
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01.04.2015
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Abstract | Miscanthus sacchariflorus 'Goedae-Uksae 1' (GU) was developed as an energy crop of high productivity in Korea. For the practical use of GU for bioethanol production, a bench-scale continuous pretreatment system was developed. The reactor performed screw extrusion, soaking and thermochemical pretreatment at the following operating conditions: 3 mm particle size, 22% moisture content, 140 °C reaction temperature, 8 min residence time, 15 g/min biomass feeding and 120 mL/min NaOH input. As a result of minimizing NaOH concentration and enzyme dosage, 90.8±0.49% glucose yield was obtained from 0.5 M NaOH-pretreated GU containing 3% glucan with 10 FPU cellulase/g cellulose at 50 °C for 72 h. The separate hydrolysis and fermentation of 0.5 M NaOH-pretreated GU containing 10% glucan with 10-30 FPU for 102 h produced 43.0-49.6 g/L bioethanol (theoretical yield, 75.8-87.6%). Thus, this study demonstrated that continuous pretreatment using a single screw reactor is effective for bioethanol production from Miscanthus biomass. |
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AbstractList | Miscanthus sacchariflorus 'Goedae-Uksae 1' (GU) was developed as an energy crop of high productivity in Korea. For the practical use of GU for bioethanol production, a bench-scale continuous pretreatment system was developed. The reactor performed screw extrusion, soaking and thermochemical pretreatment at the following operating conditions: 3 mm particle size, 22% moisture content, 140 °C reaction temperature, 8 min residence time, 15 g/min biomass feeding and 120 mL/min NaOH input. As a result of minimizing NaOH concentration and enzyme dosage, 90.8±0.49% glucose yield was obtained from 0.5 M NaOH-pretreated GU containing 3% glucan with 10 FPU cellulase/g cellulose at 50 °C for 72 h. The separate hydrolysis and fermentation of 0.5 M NaOH-pretreated GU containing 10% glucan with 10-30 FPU for 102 h produced 43.0-49.6 g/L bioethanol (theoretical yield, 75.8-87.6%). Thus, this study demonstrated that continuous pretreatment using a single screw reactor is effective for bioethanol production from Miscanthus biomass. |
Author | Ahn, Jong-Woong Park, Yuri Yang, Jungwoo An, Gi Hong Moon, Youn-Ho Yu, Gyeong-Dan Cha, Young-Lok Yoon, Young-Mi Choi, In-Hu |
Author_xml | – sequence: 1 givenname: Young-Lok surname: Cha fullname: Cha, Young-Lok organization: Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan-ro 199, Muan 534-833, Republic of Korea – sequence: 2 givenname: Jungwoo surname: Yang fullname: Yang, Jungwoo email: dbl3jwy@korea.ac.kr organization: School of Life Sciences and Biotechnology for BK21 PLUS, Korea University, Seoul 136-713, Republic of Korea. Electronic address: dbl3jwy@korea.ac.kr – sequence: 3 givenname: Yuri surname: Park fullname: Park, Yuri organization: Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan-ro 199, Muan 534-833, Republic of Korea – sequence: 4 givenname: Gi Hong surname: An fullname: An, Gi Hong organization: Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan-ro 199, Muan 534-833, Republic of Korea – sequence: 5 givenname: Jong-Woong surname: Ahn fullname: Ahn, Jong-Woong organization: Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan-ro 199, Muan 534-833, Republic of Korea – sequence: 6 givenname: Youn-Ho surname: Moon fullname: Moon, Youn-Ho organization: Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan-ro 199, Muan 534-833, Republic of Korea – sequence: 7 givenname: Young-Mi surname: Yoon fullname: Yoon, Young-Mi organization: Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan-ro 199, Muan 534-833, Republic of Korea – sequence: 8 givenname: Gyeong-Dan surname: Yu fullname: Yu, Gyeong-Dan organization: Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan-ro 199, Muan 534-833, Republic of Korea – sequence: 9 givenname: In-Hu surname: Choi fullname: Choi, In-Hu organization: Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan-ro 199, Muan 534-833, Republic of Korea |
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Keywords | Miscanthus Bioethanol Continuous pretreatment Alkaline pretreatment Lignocellulosic biomass |
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Snippet | Miscanthus sacchariflorus 'Goedae-Uksae 1' (GU) was developed as an energy crop of high productivity in Korea. For the practical use of GU for bioethanol... |
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SubjectTerms | Biomass Bioreactors Biotechnology - instrumentation Biotechnology - methods Carbohydrate Metabolism - drug effects Cellulase - metabolism Fermentation - drug effects Hydrolysis Poaceae - drug effects Sodium Hydroxide - pharmacology |
Title | Continuous alkaline pretreatment of Miscanthus sacchariflorus using a bench-scale single screw reactor |
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