Differential Microwave Resonator Sensor Reveals Glucose-Dependent Growth Profile of E. coli on Solid Agar

This letter presents a practical application of a differential microstrip microwave sensor for label-free and real-time monitoring of Escherichia coli ( E. coli ) growth under different nutritional conditions on solid agar media. Two planar microstrip ring resonators coupled with a one-port power di...

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Published inIEEE microwave and wireless components letters Vol. 30; no. 5; pp. 531 - 534
Main Authors Jain, Mandeep Chhajer, Nadaraja, Anupama Vijaya, Vizcaino, Bianca Marjan, Roberts, Deborah J., Zarifi, Mohammad H.
Format Journal Article
LanguageEnglish
Published IEEE 01.05.2020
Subjects
Bacterial growth profile
differential sensor
Frequency measurement
Glucose
glucose concentrations
growth rate
Microorganisms
Microstrip
Microwave measurement
microwave sensor
Microwave theory and techniques
Sensors
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Abstract This letter presents a practical application of a differential microstrip microwave sensor for label-free and real-time monitoring of Escherichia coli ( E. coli ) growth under different nutritional conditions on solid agar media. Two planar microstrip ring resonators coupled with a one-port power divider form the core of the sensing system. The sensing resonator operates at 1.509 GHz with an amplitude of −10.21 dB and the reference resonator operates at 1.798 GHz with an amplitude of −11.75 dB. The growth profile of E. coli at each glucose concentration in Luria-Bertani (LB) agar was derived by measuring the resonant amplitude variations (<inline-formula> <tex-math notation="LaTeX">\Delta {A} </tex-math></inline-formula>) and is further supported by microscopic images. A complete inhibition of growth was observed at 10% glucose concentration. This research expands the application of microwave sensors to investigate the effect of nutritional parameters on bacterial growth.
AbstractList This letter presents a practical application of a differential microstrip microwave sensor for label-free and real-time monitoring of Escherichia coli ( E. coli ) growth under different nutritional conditions on solid agar media. Two planar microstrip ring resonators coupled with a one-port power divider form the core of the sensing system. The sensing resonator operates at 1.509 GHz with an amplitude of −10.21 dB and the reference resonator operates at 1.798 GHz with an amplitude of −11.75 dB. The growth profile of E. coli at each glucose concentration in Luria-Bertani (LB) agar was derived by measuring the resonant amplitude variations (<inline-formula> <tex-math notation="LaTeX">\Delta {A} </tex-math></inline-formula>) and is further supported by microscopic images. A complete inhibition of growth was observed at 10% glucose concentration. This research expands the application of microwave sensors to investigate the effect of nutritional parameters on bacterial growth.
Author Roberts, Deborah J.
Jain, Mandeep Chhajer
Zarifi, Mohammad H.
Vizcaino, Bianca Marjan
Nadaraja, Anupama Vijaya
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Snippet This letter presents a practical application of a differential microstrip microwave sensor for label-free and real-time monitoring of Escherichia coli ( E....
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StartPage 531
SubjectTerms Bacterial growth profile
differential sensor
Frequency measurement
Glucose
glucose concentrations
growth rate
Microorganisms
Microstrip
Microwave measurement
microwave sensor
Microwave theory and techniques
Sensors
Title Differential Microwave Resonator Sensor Reveals Glucose-Dependent Growth Profile of E. coli on Solid Agar
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Volume 30
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