Effect of Protein Oxidation on Microbial, Free Amino Acids and Biogenic Amine Content in Chinese Dry Sausage

• Published in: Current topics in nutraceuticals research Vol. 17; no. 2; pp. 188 - 194
• Main Authors: Zhao, Bing, Li, Su, Zhou, Hui-min, Zhang, Shun-liang, Wang, Hui, Wang, Shou-wei
• Format: Journal Article
• Language: English
• Published: New Century Health Publishers, LLC 01.05.2019
• Subjects: Amines; Amino acids; Biogenic amines; Catecholamines; Chromatography; Email; Ethylene glycols; Glycols (Class of compounds); High performance liquid chromatography; Liquid chromatography; Meat; Meat industry; Oxidation-reduction reactions; Proteins; Reagents; Technology
• DOI: 10.37290/ctnr2641-452x.17:188-194
• ISSN: 1540-7535

The objective of this study was to investigate the effect of protein oxidation on microbial, free amino acids (FAAs) and biogenic amines (BAs) in Chinese dry sausage during processing. Three different batches of Chinese dry sausages were examined: control batch, pro-oxidation batch and anti-oxidation batch. The experimental results revealed that anti-oxidation batch showed the lowest degree of protein oxidation compared with control batch, while the results of the pro-oxidation batch were just opposite. With the increasing protein oxidation, estimated by protein carbonyls and sulfhydryls, microbial content and free amino acid content increased, while BA content decreased. The protein carbonyls were positively correlated with total FAAs (P<0.01) and total BA content (P<0.05), while sulfhydryls content was negatively correlated with total FAA content (P<0.01), total viable counts (P<0.01), and total BA content (P<0.01). In conclusion, reduced protein oxidation contributes to improved quality of Chinese dry sausage. This research provided a new strategy to improve the quality of meat product manufacture. Keywords: Biogenic amine; Chinese dry sausage; Free amino acid; Myofibril; Proteins oxidation Corresponding Author: Shou-wei Wang, Beijing Academy of Food Sciences, China Meat Research Centre, Beijing Key Laboratory of Meat Processing Technology, Beijing 100068, China; Fax: +86-010-87293157; E-mail: cmrcwang@163.com Abbreviations Used: Anti-oxidation batch, AO; Biogenic amine, BA; Control batch, C; Colony forming unit, CFU; 2,4-Dinitrophenylhydrazine, DNPH; 5,5'-Dithiobis (2-nitrobenzoic acid), DTNB; Ethylene glycol-bis (P-aminoethyl ether)-N, N, N', N'-tetraacetic acid, EGTA; Free amino acids, FAAs; High pressure liquid chromatography, HPLC; Pro-oxidation batch, PO; Reversed phase high pressure liquid chromatography, RP-HPLC