A novel approach to quantitating leukemia fusion transcripts by qRT-PCR without the need for standard curves

• Published in: Experimental and molecular pathology Vol. 99; no. 1; pp. 104 - 108
• Main Authors: Schumacher, Jonathan A., Scott Reading, N., Szankasi, Philippe, Matynia, Anna P., Kelley, Todd W.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.08.2015
• Subjects: Acute myeloid leukemia; Chromosome Aberrations; Core Binding Factor beta Subunit - genetics; Core Binding Factor beta Subunit - metabolism; Cost-Benefit Analysis; DNA Fragmentation; Fusion transcripts; Humans; inv; Leukemia, Myeloid, Acute - diagnosis; Leukemia, Myeloid, Acute - genetics; Molecular testing; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Oncogene Proteins, Fusion - genetics; Oncogene Proteins, Fusion - metabolism; Plasmids - genetics; Polymerase chain reaction; Polymerase Chain Reaction - methods; Promyelocytic Leukemia Protein; RNA, Messenger - genetics; RNA, Messenger - metabolism; Specimen Handling; t(15;17); Transcription Factors - genetics; Transcription Factors - metabolism; Tumor Suppressor Proteins - genetics; Tumor Suppressor Proteins - metabolism; inv; Polymerase chain reaction; Fusion transcripts; Molecular testing; t(15;17); Acute myeloid leukemia
• ISSN: 0014-4800; 1096-0945
• DOI: 10.1016/j.yexmp.2015.06.006

Acute myeloid leukemia patients with recurrent cytogenetic abnormalities including inv(16);CBFB-MYH11 and t(15;17);PML-RARA may be assessed by monitoring the levels of the corresponding abnormal fusion transcripts by quantitative reverse transcription-PCR (qRT-PCR). Such testing is important for eva...