Double stranded RNA in heterogeneous nuclear RNA from normal and chronic lymphocytic leukemic lymphocytes

• Published in: Cancer letters Vol. 4; no. 2; pp. 99 - 107
• Main Authors: Mnsson, Per Erik, Pero, Ronald W., Brandt, Lars
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.01.1978
• Subjects: Humans; Leukemia, Lymphoid - analysis; Lymphocytes - analysis; Poly A - analysis; RNA - analysis; RNA, Neoplasm - analysis; dsRNA; chronic lymphocytic leukemic; CLL; white blood cell; messenger RNA; HnRNA; WBC; mRNA; heterogeneous nuclear RNA; double stranded RNA
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/S0304-3835(78)93742-4

Tritium labelled heterogeneous nuclear RNA (HnRNA) from normal and chronic lymphocytic leukemic (CLL) lymphocytes was investigated before and after fractionation into non-poly(A) containing (−HnRNA) and poly(A) containing (+HnRNA) HnRNA with respect to double stranded RNA (dsRNA). Statistically significant higher amounts of rapidly labelled RNA were recovered from CLL lymphocytes when compared to normal cases. Within the CLL cases a significant linear correlation ( r = 0.95) was found between white blood cell counts and the amount of dsRNA in total HnRNA. After fractionation into (−) and (+) HnRNAs the ratios of dsRNAs, expressed as the dsRNA in (−) HnRNA divided by the dsRNA in (+) HnRNA, was lower than the corresponding values in normal cases for all the CLL cases except one. The relationship between (+) HnRNA and the total dsRNA level was different when comparing CLL and normal lymphocytes indicating a RNA processing abnormality.