Cohesin supercoils DNA during loop extrusion

• Published in: Cell reports (Cambridge) Vol. 44; no. 6; p. 115856
• Main Authors: Davidson, Iain F., Barth, Roman, Nagasaka, Kota, Tang, Wen, Wutz, Gordana, Horn, Sabrina, Janissen, Richard, Stocsits, Roman R., Chlosta, Emilia, Bauer, Benedikt W., Dekker, Cees, Peters, Jan-Michael
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 24.06.2025
• Subjects: Cell Cycle Proteins - chemistry; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Chromosomal Proteins, Non-Histone - chemistry; Chromosomal Proteins, Non-Histone - genetics; Chromosomal Proteins, Non-Histone - metabolism; cohesin; Cohesins; DNA, Superhelical - chemistry; DNA, Superhelical - genetics; DNA, Superhelical - metabolism; loop extrusion; Nucleic Acid Conformation; SMC complexes; supercoiling; topoisomerases; CP: Molecular biology; topoisomerases; cohesin; SMC complexes; loop extrusion; supercoiling

Cohesin extrudes genomic DNA into loops that promote chromatin assembly, gene regulation, and gene recombination. Loop extrusion depends on large-scale conformational changes in cohesin, but how these translocate DNA is poorly understood. Here, we provide evidence that cohesin negatively supercoils...