Description of Helicobacter valdiviensis sp. nov., an Epsilonproteobacteria isolated from wild bird faecal samples

• Published in: International journal of systematic and evolutionary microbiology Vol. 64; no. Pt 6; pp. 1913 - 1919
• Main Authors: Collado, Luis, Jara, Ronald, González, Susana
• Format: Journal Article
• Language: English
• Published: England International Union of Microbiological Societies 01.06.2014
• Subjects: Animals; Bacterial Typing Techniques; Birds - microbiology; Chaperonin 60 - genetics; Chile; DNA Gyrase - genetics; DNA, Bacterial - genetics; feces; Feces - microbiology; genes; heat shock proteins; Helicobacter - classification; Helicobacter - genetics; Helicobacter - isolation & purification; Helicobacter canadensis; Helicobacter pametensis; Helicobacter pullorum; microbiology; Molecular Sequence Data; nucleotide sequences; Phylogeny; polymerase chain reaction; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; sequence homology; wild birds; Chile
• ISSN: 1466-5034; 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.057141-0

Two Gram-stain-negative, gently curved rod-shaped isolates (WBE14T and WBE19), recovered from wild bird faecal samples in the city of Valdivia (Southern Chile) were subjected to a polyphasic taxonomic study. Results of a genus-specific PCR indicated that these isolates belonged to the genus Helicobacter. This was further confirmed by a phylogenetic analyses based on the 16S rRNA, 60 kDa heat-shock protein (cpn60) and gyrase subunit B (gyrB) genes, where both strains formed a novel phylogenetic line within this genus. The 16S rRNA gene sequence similarity of strain WBE14T to the type strains of all other species of the genus Helicobacter examined ranged from 89.4 to 97.0 %; Helicobacter brantae and Helicobacter pametensis were the most closely related species. However, on the basis of the protein-coding genes Helicobacter pullorum and Helicobacter canadensis are the most closely related species. These data, together with their different morphological and biochemical characteristics, revealed that these strains represent a novel species, for which the name Helicobacter valdiviensis sp. nov. is proposed, with the type strain WBE14T ( = CECT 8410T = LMG 27920T).