Molecular identification of root fungal associates in Orchis pauciflora Tenore

The terrestrial orchid, Orchis pauciflora Ten., growing in poor grassland and garrigue of Central Mediterranean region, is local and rare and has been included in the red lists of several Italian regions. We investigated the diversity of fungal associates in O. pauciflora adult plants collected in t...

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Published inPlant biosystems Vol. 146; no. 4; pp. 985 - 991
Main Authors Pecoraro, L, Girlanda, M, Kull, T, Perini, C, Perotto, S
Format Journal Article
LanguageEnglish
Published Abingdon Taylor & Francis 01.12.2012
Taylor & Francis Ltd
Subjects
Ascomycota
Basidiomycota
conservation
conservation areas
DNA
Exophiala
fungal symbionts
fungi
grasslands
internal transcribed spacers
mature plants
Orchidaceae
Orchis
Phialophora
roots
trophic relationships
Tulasnellaceae
Mediterranean region
Italy
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Abstract The terrestrial orchid, Orchis pauciflora Ten., growing in poor grassland and garrigue of Central Mediterranean region, is local and rare and has been included in the red lists of several Italian regions. We investigated the diversity of fungal associates in O. pauciflora adult plants collected in two protected areas of Tuscany (Central Italy). Genomic DNA was extracted from mycorrhizal roots of 12 orchid plants and the fungal ITS were amplified and sequenced. Several fungal associates, belonging to different taxa of basidiomycetes (Tulasnellaceae) and ascomycetes such as Leptodontidium, Exophiala and Phialophora species, were recovered. The trophic role of these fungi and their impact on O. pauciflora growth and conservation are discussed.
AbstractList The terrestrial orchid, Orchis pauciflora Ten., growing in poor grassland and garrigue of Central Mediterranean region, is local and rare and has been included in the red lists of several Italian regions. We investigated the diversity of fungal associates in O. pauciflora adult plants collected in two protected areas of Tuscany (Central Italy). Genomic DNA was extracted from mycorrhizal roots of 12 orchid plants and the fungal ITS were amplified and sequenced. Several fungal associates, belonging to different taxa of basidiomycetes (Tulasnellaceae) and ascomycetes such as Leptodontidium, Exophiala and Phialophora species, were recovered. The trophic role of these fungi and their impact on O. pauciflora growth and conservation are discussed. [PUBLICATION ABSTRACT]
The terrestrial orchid, Orchis pauciflora Ten., growing in poor grassland and garrigue of Central Mediterranean region, is local and rare and has been included in the red lists of several Italian regions. We investigated the diversity of fungal associates in O. pauciflora adult plants collected in two protected areas of Tuscany (Central Italy). Genomic DNA was extracted from mycorrhizal roots of 12 orchid plants and the fungal ITS were amplified and sequenced. Several fungal associates, belonging to different taxa of basidiomycetes (Tulasnellaceae) and ascomycetes such as Leptodontidium, Exophiala and Phialophora species, were recovered. The trophic role of these fungi and their impact on O. pauciflora growth and conservation are discussed.
Author Kull, T
Perini, C
Pecoraro, L
Perotto, S
Girlanda, M
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SubjectTerms Ascomycota
Basidiomycota
conservation
conservation areas
DNA
Exophiala
fungal symbionts
fungi
grasslands
internal transcribed spacers
mature plants
Orchidaceae
Orchis
Phialophora
roots
trophic relationships
Tulasnellaceae
Title Molecular identification of root fungal associates in Orchis pauciflora Tenore
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