Fluorochrome‐dependent specific changes in spectral profiles using different compensation beads or primary cells in full spectrum cytometry

• Published in: Cytometry. Part A Vol. 105; no. 6; pp. 458 - 463
• Main Authors: Shevchenko, Yaroslava, Lurje, Isabella, Tacke, Frank, Hammerich, Linda
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.06.2024; Wiley Subscription Services, Inc
• Subjects: Animals; Compensation; compensation beads; Flow cytometry; Flow Cytometry - methods; Fluorescent Dyes - chemistry; Fluorophores; full spectrum flow cytometry; Humans; Leukocytes; Leukocytes - cytology; Leukocytes - metabolism; Mice; Microspheres; reference controls; Spectral signatures; spectrum overlay; compensation beads; reference controls; spectrum overlay; full spectrum flow cytometry

Full spectrum flow cytometry is a powerful tool for immune monitoring on a single‐cell level and with currently available machines, panels of 40 or more markers per sample are possible. However, with an increased panel size, spectral unmixing issues arise, and appropriate single stain reference cont...