Determination of isoniazid concentration in rabbit vertebrae by isotope tracing technique in conjunction with HPLC

• Published in: Biomedical chromatography Vol. 27; no. 9; pp. 1150 - 1156
• Main Authors: Liu, Peng, Fu, Zhaozong, Jiang, Jianming, Yuan, Liang, Lin, Zhen
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.09.2013
• Subjects: Animals; Antitubercular Agents - analysis; Antitubercular Agents - blood; Antitubercular Agents - pharmacokinetics; Chromatography, High Pressure Liquid - methods; drug metabolism; Female; HPLC; isoniazid; Isoniazid - analysis; Isoniazid - blood; Isoniazid - pharmacokinetics; Linear Models; Male; Rabbits; Radionuclide Imaging; spinal tuberculosis; Spine - chemistry; Spine - diagnostic imaging; Technetium - analysis; Technetium - blood; Technetium - pharmacokinetics; technetium-99; Tissue Distribution; Whole Body Imaging; isoniazid; spinal tuberculosis; HPLC; technetium-99; drug metabolism
• DOI: 10.1002/bmc.2920
• ISSN: 0269-3879

ABSTRACT Medications compounded with isoniazid (INH) are usually applied to surgical sites at the completion of surgery to locally kill postoperative residual tubercle bacilli. However, the distribution and elimination of INH in the vertebrae in vivo are not known. In this study, isotope tracing was used in conjunction with high‐pressure liquid chromatography (HPLC) to address this. INH and technetium‐99 m‐labeled INH were applied to the vertebrae of rabbits. After 2 and 6 h, osseous tissues containing INH, as determined by radionuclide imaging, were collected for detection with HPLC. The results showed that INH mainly stayed around the vertebrae 6 h after its application and did not permeate widely into the blood or other organs, except for the kidneys. The standard deviations of INH concentrations in the technetium‐99 m‐INH group were approximately four‐fold smaller than those in the INH group. This method of coupling isotope tracing and HPLC can effectively limit experimental error during sample collection, allowing accurate and reliable identification of the concentration levels of INH in osseous tissues in vivo. Copyright © 2013 John Wiley & Sons, Ltd.