Further development of Axisymmetric Drop Shape Analysis-Captive Bubble for pulmonary surfactant related studies

• Published in: Biochimica et biophysica acta Vol. 1675; no. 1; pp. 12 - 20
• Main Authors: Zuo, Y.Y., Ding, M., Li, D., Neumann, A.W.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 18.11.2004
• Subjects: Adsorption; Animals; Axisymmetric Drop Shape Analysis (ADSA); Captive Bubble (CB); Cattle; Dynamic surface tension measurement; Image analysis; Image Processing, Computer-Assisted; Phospholipids - metabolism; Pulmonary surfactant; Pulmonary Surfactants - chemistry; Surface Tension; Captive Bubble (CB); Image analysis; Dynamic surface tension measurement; Pulmonary surfactant; Axisymmetric Drop Shape Analysis (ADSA)
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/j.bbagen.2004.08.003

The methodology combining Axisymmetric Drop Shape Analysis (ADSA) with a captive bubble (ADSA-CB) facilitates pulmonary surfactant related studies. The accuracy of ADSA-CB is crucially dependent on the quality of the bubble profile extracted from the raw image. In a previous paper, an image analysis scheme featuring a Canny edge detector and a Axisymmetric Liquid Fluid Interfaces-Smoothing (ALFI-S) algorithm was developed to process captive bubble images under a variety of conditions, including images with extensive noise and/or lack of contrast. A new version of ADSA-CB based on that image analysis scheme is developed and applied to pulmonary surfactant and pulmonary surfactant–polymer systems. The new version is found to be highly noise-resistant and well self-adjusting.