Immunomodulation by extracellular vesicle-like nanoparticles from marine macroalgae Sargassum fusiforme: Enhancing Type 1 T helper and cytotoxic T lymphocyte-mediated immune responses

• Published in: Journal of functional foods Vol. 112; p. 105981
• Main Authors: Lee, Hyeon Jin, Shin, Ki-Won, Lee, Seo Jun, Park, Ji Young, Lee, In Chul, Kwon, Hyung-Jun, Jeong, Hyung Jae, Yuk, Jae-Min, Ryu, Young-Bae, Kim, Woo Sik
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.01.2024; Elsevier
• Subjects: Cytotoxic T lymphocyte; Dendritic cells; Immune restoration; Immunosuppression mouse model; Marine macroalgae-derived nanoparticles; Th1 polarization; CTX; DLS; Q-TOF-MS; TNF; LPS; Immunosuppression mouse model; NF-κB; RBC; SF; TFF; Th1 polarization; MSC; Th1; Cytotoxic T lymphocyte; Th17; Th2; PBS; Immune restoration; IL; Dendritic cells; SF-NPs; CFSE; MAPK; EV; PC; PE; UPLC; PI; CTL; SEM; Marine macroalgae-derived nanoparticles; OVA; NK; DC
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2023.105981

[Display omitted] •SF-NPs stimulate the maturation of DCs through the MAPK and NF-kB signaling pathways.•SF-NPs-primed DCs drive robust Th1 dominance and cytotoxic T lymphocyte responses.•SF-NPs restored innate and adaptive responses in immunocompromised conditions.•SF-NPs exhibit therapeutic potential for diseases requiring Th1 and CTL responses. This study investigated the immunological features and therapeutic potential of extracellular vesicle-like nanoparticles isolated from the marine macroalgae Sargassum fusiforme (SF-NPs). SF-NPs were approximately 266.4 nm in size, and comprehensive metabolomic analysis revealed 38 metabolites. SF-NPs can upregulate dendritic cell (DC) maturation markers, increase pro-inflammatory cytokine production, and enhance antigen presentation capacity. DC maturation is regulated by the mitogen-activated protein kinase and nuclear factor-kappa B pathways. Notably, SF-NPs-primed DCs orchestrated robust CD4+ and CD8+ T-cell proliferation, fostering potent Type 1 T-helper(Th1) responses and cytotoxic T lymphocyte (CTL) activity. In a cyclophosphamide-induced immunosuppression mouse model, SF-NPs restored and activated immune cell populations, including DCs, natural killer, CD4+ T, and CD8+ T cells. SF-NPs reversed cyclophosphamide-induced Type 2 T-helperand regulatory T cell-biased responses, favoring Th1 and CTL responses, including multifunctional T cell responses. This study highlighted the potential of SF-NPs as therapeutic candidates for diseases requiring Th1 and CTL responses.