The use of granulocyte colony-stimulating factor during retroviral transduction on fibronectin fragment CH-296 enhances gene transfer into hematopoietic repopulating cells in dogs

• Published in: Blood Vol. 94; no. 7; pp. 2287 - 2292
• Main Authors: GOERNER, M, BRUNO, B, MCSWEENEY, P. A, BURON, G, STORB, R, KIEM, H.-P
• Format: Journal Article
• Language: English
• Published: Washington, DC The Americain Society of Hematology 01.10.1999
• Subjects: Animals; Antigens, CD34 - analysis; Biological and medical sciences; Biotechnology; Bone Marrow Cells - cytology; Cattle; Coculture Techniques; Dogs; Fibronectins - pharmacology; Fundamental and applied biological sciences. Psychology; Gene therapy; Gene Transfer Techniques; Genetic Therapy - methods; Genetic Vectors; Growth Substances - pharmacology; Health. Pharmaceutical industry; Hematopoietic Stem Cell Transplantation; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - drug effects; Hematopoietic Stem Cells - physiology; Humans; Industrial applications and implications. Economical aspects; Membrane Proteins - pharmacology; Peptide Fragments - pharmacology; Stem Cell Factor - pharmacology; Thrombopoietin - pharmacology; Transfection - methods; Flt3 ligand; Stem cell; Hematopoietic cell; Fibronectin; Thrombopoietin; Granulocyte colony stimulating factor; Gene; Bone marrow; Mast cell growth factor; Dog; Vector; Fissipedia; Carnivora; Cytokine; Retroviridae; Genetic transfer; Virus; Vertebrata; Mammalia; Polypeptide; Peptide fragment; Animal; Genetic engineering; Transduction; Gene therapy; Growth factor

A competitive repopulation assay in the dog was used to develop improved gene transfer protocols for hematopoietic stem cell gene therapy. Using this assay, we previously showed improved gene transfer into canine hematopoietic repopulating cells when CD34-enriched marrow cells were cocultivated on g...