Comparison of two total cellular fatty acid analysis protocols to differentiate Rhizoctonia oryzae and R. oryzae-sativae

• Published in: Mycologia Vol. 97; no. 1; pp. 77 - 83
• Main Authors: Lanoiselet, V.M, Cother, E.J, Cother, N.J, Ash, G.J, Harper, J.D.I
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 2005; Mycological Society of America
• Subjects: Acid soils; Anastomosis groups; Bacteria; Ceratobasidium oryzae-sativae; Chemical composition; FAME; fatty acid composition; Fatty acids; Fatty Acids - analysis; Fungi; host plants; identification; Methods; MIDI; Mycelium; Mycological Typing Techniques; Oryza - microbiology; Oryza sativa; Plant Diseases - microbiology; plant pathogenic fungi; Plants; provenance; Rhizoctonia; Rhizoctonia - chemistry; Rhizoctonia - classification; Rhizoctonia oryzae; Rhizoctonia oryzae-sativae; Rice; Waitea oryzae; Australia; United States; Uruguay; Japan

Two fatty acid analysis protocols (the MIDI and a modified MIDI method) were investigated for their utility to characterize and differentiate Rhizoctonia oryzae and R. oryzae-sativae isolates from four countries. Only the modified MIDI method permitted a clear differentiation between the two species...