Simultaneous Determination and Pharmacokinetic Study of Metformin and Rosiglitazone in Human Plasma by HPLC-ESI-MS

• Published in: Journal of chromatographic science Vol. 49; no. 2; pp. 94 - 100
• Main Authors: Chen, Lingyun, Zhou, Zhifeng, Shen, Mei, Ma, Ande
• Format: Journal Article
• Language: English
• Published: Niles, IL Oxford University Press 01.02.2011; Preston Publications
• Subjects: Analysis; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Drug Stability; Female; General pharmacology; Humans; Least-Squares Analysis; Male; Medical sciences; Metformin - blood; Metformin - chemistry; Metformin - pharmacokinetics; Pharmacology. Drug treatments; Phenformin - analysis; Reproducibility of Results; Rosiglitazone; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization - methods; Thiazolidinediones - blood; Thiazolidinediones - chemistry; Thiazolidinediones - pharmacokinetics; Human; Validation; Biological fluid; Tandem mass spectrometry; Chemical analysis; Healthy subject; Coupled method; Oral administration; HPLC chromatography; Thiazolidinedione derivatives; Electrospray; Blood; Blood plasma; Hypoglycemic agent; Biguanides; Simultaneous measurement; Rosiglitazone; Metformin; Pharmacokinetics; Quantitative analysis
• DOI: 10.1093/chrsci/49.2.94
• ISSN: 0021-9665; 1945-239X

A fast, sensitive and specific high-performance liquid chromatography tandem mass spectrometry method was develope for simultaneous determination of metformin and rosiglitazone in human plasma. With phenformin as an internal standard, the analysis was carried out on a C18 column (50 mm × 2.1 mm, 3.5 µm) using a mobile phase consisting of acetonitrile-10 mM ammonium acetate (20:80, v/v). The detection was performed by tandem mass spectrometry via electrospray ionization. Linear calibration curves were obtained in the concentration of 1.054-263.5 ng/mL for rosiglitazone and 4.040-5050 ng/mL for metformin. The method was applicable to clinical pharmacokineti study of metformin and rosiglitazone in healthy volunteers following oral administration.