A novel electrochemical DNA-sensing nanoplatform based on supramolecular ionic liquids grafted on nitrogen-doped graphene aerogels

• Published in: Journal of applied electrochemistry Vol. 45; no. 12; pp. 1289 - 1298
• Main Authors: Kazerooni, Hanif, Nasernejad, Bahram
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.12.2015
• Subjects: Chemistry; Chemistry and Materials Science; Electrochemistry; Industrial Chemistry/Chemical Engineering; Physical Chemistry; Research Article; Sensors and Electrochemical Methods; Nucleic acids; Biosensors; Polymers; Nanotechnology; Electrochemical sensors
• ISSN: 0021-891X; 1572-8838
• DOI: 10.1007/s10800-015-0891-3

In this paper, the application of supramolecular ionic liquids grafted on nitrogen-doped graphene aerogels (SIL-g-(N)GAs), a novel electrode system, for the preparation of electrochemical DNA sensing platform is proposed. The super dispersion of SIL-g-(N)GAs in water makes it an ideal candidate for biological purposes such as gene delivery. In fact, SIL-g-(N)GAs/glassy carbon working electrode (GCE) can realize the simultaneous detection of all four DNA bases in double-stranded DNA without a prehydrolysis step. On the SIL-g-(N)GAs/GCE, due to the presence of SIL on the surface of three-dimensional nitrogen-doped graphene, the anchoring of the DNA probe can be achieved by electrostatic association of SIL cations with the DNA backbone readily. So, herein we report a novel strategy for DNA hybridization without any electroactive tags or intercalators and suggest the potential applications of SIL-g-(N)GAs/GCE in the label-free electrochemical detection of DNA hybridization or DNA damage for further research. Moreover, the influence of potentially interfering substances on the determination of DNA is investigated. Graphical Abstract