Protein-carbohydrate interaction I. The interaction of polysaccharides with concanavalin A

The precipitin reaction between concanavalin A, a globulin isolated from jackbean meal, and certain polysaccharides has been examined. Using a turbidimetric assay and a partially purified concanavalin A preparation, it has been shown that only branched polysaccharides possessing α- d-glucopyranosyl...

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Published inBiochimica et biophysica acta Vol. 97; no. 1; pp. 68 - 76
Main Authors Goldstein, I.J., Hollerman, C.E., Merrick, J.M.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 04.01.1965
Subjects
Biochemical Phenomena
Biochemistry
Concanavalin A
Dextrans
Globulins
Liver Glycogen
OldMedline
Plant Proteins
Polysaccharides
Precipitin Tests
Saccharomyces
PLANT PROTEINS
PRECIPITIN TESTS
BIOCHEMISTRY
LIVER GLYCOGEN
DEXTRAN
EXPERIMENTAL LAB STUDY
POLYSACCHARIDES
SACCHAROMYCES
GLOBULINS
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Summary:The precipitin reaction between concanavalin A, a globulin isolated from jackbean meal, and certain polysaccharides has been examined. Using a turbidimetric assay and a partially purified concanavalin A preparation, it has been shown that only branched polysaccharides possessing α- d-glucopyranosyl or α- d-mannopyranosyl units will interact with the protein to form a precipitate. The extent of precipitate formation is dependent on the concentration of both concanavalin A and polysaccharide and the reaction appears to be analogous to an antibody-antigen system. The frequency of branching of the polysaccharide molecule plays an important role in the extent of interaction with concanavalin A. Dextrans may be classified according to their extent of reaction with the protein.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/0304-4165(65)90270-9