Cloning of CHO Cells, Productivity and Genetic Stability—A Discussion

• Published in: Processes Vol. 5; no. 4; p. 20
• Main Authors: Wurm, Florian, Wurm, Maria
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.06.2017
• Subjects: Biological evolution; Bioreactors; Cell culture; Cell division; Cloning; Division; Evolution; Fitness; Genetic diversity; Genomes; Genomic instability; Manufacturing; Mutation rates; Population genetics; Populations; Progeny; Replication; Reproductive fitness; Stability; Transfection; Trends

While many perceive mammalian cell culture-based manufacturing for biopharmaceuticals an established technology, numerous open questions remain to be solved. Genetic diversity and mutation rates in CHO cells have been underestimated since progeny of a clonal CHO cell become genetically diverse with...