AMPA receptor imaging by infrared scanning near-field optical microscopy
Infrared scanning near‐field optical microscopy is used to detect AMPA‐type glutamate receptor clusters in hippocampal neuron cells. AMPA's GluR2‐subunits were marked with the fluorophore Alexa488 and high‐resolution micrographs were taken at 6.25 µm wavelength, corresponding to the maker'...
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Published in | Physica status solidi. C Vol. 5; no. 8; pp. 2641 - 2644 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin
WILEY-VCH Verlag
01.06.2008
WILEY‐VCH Verlag |
Subjects | |
Online Access | Get full text |
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Summary: | Infrared scanning near‐field optical microscopy is used to detect AMPA‐type glutamate receptor clusters in hippocampal neuron cells. AMPA's GluR2‐subunits were marked with the fluorophore Alexa488 and high‐resolution micrographs were taken at 6.25 µm wavelength, corresponding to the maker's strong infrared absorption. The use of infrared light avoids photobleaching and makes this approach suitable for long‐term observations; this technique can be used in general to localize different IR‐absorbing molecules over the complex background of other cell components. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) |
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Bibliography: | Fonds National Suisse de la Recherche Scientifique - No. 200020-113047; No. 3100A0-111935 Vanderbilt University Free Electron Laser Center ArticleID:PSSC200779116 Italian National Research Council istex:FC949ABFBE730FFB33E2161C40EA237AAC371618 under the Department of Defense MFEL Program ark:/67375/WNG-FPBN2NMF-Q ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 1862-6351 1610-1634 1610-1642 |
DOI: | 10.1002/pssc.200779116 |