Combining non-canonical amino acid mutagenesis and native chemical ligation for multiply modifying proteins: A case study of α-synuclein post-translational modifications

• Published in: Methods (San Diego, Calif.) Vol. 218; pp. 101 - 109
• Main Authors: Galesic, Ana, Pan, Buyan, Ramirez, Jennifer, Rhoades, Elizabeth, Pratt, Matthew R., Petersson, E. James
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2023
• Subjects: alpha-Synuclein - chemistry; alpha-Synuclein - genetics; alpha-Synuclein - metabolism; Amino Acids - metabolism; case studies; fluorescence correlation spectroscopy; fluorescent dyes; genetic code; Humans; Mutagenesis; neurotransmitters; Parkinson Disease - genetics; phosphorylation; Protein Processing, Post-Translational; serine

•We combine genetic code expansion and native chemical ligation to modify the Parkinson’s disease associated protein α-synuclein.•We study the effects of O-linked β-N-acetylglucosamine modification or phosphorylation of serine 87 on the membrane binding of α-synuclein.•Beyond post-translational modi...