Augmentation of murine natural killer activity after culture
The augmentation of murine natural killer (NK) cell activity after culture is described. Increased NK cell activity occurred when spleen cells were cultured for 18 or 42 h at high cell density in macro culture plates at 37 degrees C. Similar results were also achieved using the same cell density whe...
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Published in | Journal of immunological methods Vol. 48; no. 3; p. 359 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
01.01.1982
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Abstract | The augmentation of murine natural killer (NK) cell activity after culture is described. Increased NK cell activity occurred when spleen cells were cultured for 18 or 42 h at high cell density in macro culture plates at 37 degrees C. Similar results were also achieved using the same cell density when micro culture plates were employed. The simple modifications of culture conditions described in this paper should provide an excellent tool to study murine NK activity after culture. Furthermore, the micro culture system has the added advantage of enabling one to test large numbers of samples. |
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AbstractList | The augmentation of murine natural killer (NK) cell activity after culture is described. Increased NK cell activity occurred when spleen cells were cultured for 18 or 42 h at high cell density in macro culture plates at 37 degrees C. Similar results were also achieved using the same cell density when micro culture plates were employed. The simple modifications of culture conditions described in this paper should provide an excellent tool to study murine NK activity after culture. Furthermore, the micro culture system has the added advantage of enabling one to test large numbers of samples. |
Author | Kaminski, D A Cohen, S A Salazar, D |
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SubjectTerms | Animals Cells, Cultured Concanavalin A - pharmacology Cytotoxicity Tests, Immunologic - methods Female Lipopolysaccharides - pharmacology Mice Mice, Inbred C3H Spleen - cytology Spleen - immunology |
Title | Augmentation of murine natural killer activity after culture |
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