Augmentation of murine natural killer activity after culture

The augmentation of murine natural killer (NK) cell activity after culture is described. Increased NK cell activity occurred when spleen cells were cultured for 18 or 42 h at high cell density in macro culture plates at 37 degrees C. Similar results were also achieved using the same cell density whe...

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Published inJournal of immunological methods Vol. 48; no. 3; p. 359
Main Authors Cohen, S A, Salazar, D, Kaminski, D A
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.1982
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Abstract The augmentation of murine natural killer (NK) cell activity after culture is described. Increased NK cell activity occurred when spleen cells were cultured for 18 or 42 h at high cell density in macro culture plates at 37 degrees C. Similar results were also achieved using the same cell density when micro culture plates were employed. The simple modifications of culture conditions described in this paper should provide an excellent tool to study murine NK activity after culture. Furthermore, the micro culture system has the added advantage of enabling one to test large numbers of samples.
AbstractList The augmentation of murine natural killer (NK) cell activity after culture is described. Increased NK cell activity occurred when spleen cells were cultured for 18 or 42 h at high cell density in macro culture plates at 37 degrees C. Similar results were also achieved using the same cell density when micro culture plates were employed. The simple modifications of culture conditions described in this paper should provide an excellent tool to study murine NK activity after culture. Furthermore, the micro culture system has the added advantage of enabling one to test large numbers of samples.
Author Kaminski, D A
Cohen, S A
Salazar, D
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Snippet The augmentation of murine natural killer (NK) cell activity after culture is described. Increased NK cell activity occurred when spleen cells were cultured...
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StartPage 359
SubjectTerms Animals
Cells, Cultured
Concanavalin A - pharmacology
Cytotoxicity Tests, Immunologic - methods
Female
Lipopolysaccharides - pharmacology
Mice
Mice, Inbred C3H
Spleen - cytology
Spleen - immunology
Title Augmentation of murine natural killer activity after culture
URI https://www.ncbi.nlm.nih.gov/pubmed/7061869
Volume 48
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