Drug-induced residual damage of murine hematopoietic stem cells measured by a new assay

• Published in: Leukemia research Vol. 6; no. 6; pp. 815 - 818
• Main Authors: Hübner, Günter E., Cronkite, Eugene P.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 1982
• Subjects: 125iodo-deoxyuridine; Animals; busulfan and cyclophosphamide; Cell Division - drug effects; Female; Hematopoietic stem cells; Hematopoietic Stem Cells - drug effects; Hematopoietic Stem Cells - metabolism; Idoxuridine - metabolism; intracellular residual damage; Iodine Radioisotopes; Male; methylnitrosourea; Methylnitrosourea - pharmacology; Mice; Mice, Inbred C57BL; 125 IUdR; BU; MNU; 125iodo-deoxyuridine; intracellular residual damage; busulfan and cyclophosphamide; methylnitrosourea; CP; Hematopoietic stem cells

A recently developed assay for the measurement of residual stem cell damage after gamma-irradiation was tested to see if it could detect residual drug-induced stem cell damage. In this assay, the proliferative ability of transfused donor bone marrow is determined in the spleens of lethally irradiate...