Use of carboxypeptidase A for simultaneous assessment of purity and assignment of human Bence Jones proteins and light chains to K and L classes
A simple and rapid method involving the use of carboxypeptidase A was developed to assess the purity of human Bence Jones proteins and light chains and simultaneously to assign them to their principal antigenic class (K- or L-type). Only serine was released from purified L-type proteins by the actio...
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| Published in | Archives of biochemistry and biophysics Vol. 132; no. 2; pp. 502 - 508 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Elsevier Inc
01.07.1969
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| Subjects | |
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| Abstract | A simple and rapid method involving the use of carboxypeptidase A was developed to assess the purity of human Bence Jones proteins and light chains and simultaneously to assign them to their principal antigenic class (K- or L-type). Only serine was released from purified L-type proteins by the action of the enzyme, while no amino acids were liberated from K-type proteins. The heavy chain of a human myeloma IgG protein was also not susceptible to hydrolysis, but serine was released from the L-type light chain of the intact myeloma protein. |
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| AbstractList | A simple and rapid method involving the use of carboxypeptidase A was developed to assess the purity of human Bence Jones proteins and light chains and simultaneously to assign them to their principal antigenic class (K- or L-type). Only serine was released from purified L-type proteins by the action of the enzyme, while no amino acids were liberated from K-type proteins. The heavy chain of a human myeloma IgG protein was also not susceptible to hydrolysis, but serine was released from the L-type light chain of the intact myeloma protein. |
| Author | Sheber, Florence A. Simonds, Nanne B. Edmundson, Allen B. Johnson, Timothy Bangs, Betty |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/5797336$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1021/ac60139a006 10.1016/S0065-3233(08)60022-4 10.1042/bj0990356 10.1126/science.149.3688.1090 10.1016/S0021-9258(19)83985-6 10.1016/0003-9861(68)90283-X 10.1038/212205a0 10.1152/physrev.1957.37.4.512 10.1016/S0021-9258(19)67917-2 10.1016/S0021-9258(18)99854-6 10.1016/S0065-2776(08)60126-1 10.1016/S0021-9258(19)83983-2 10.1016/S0022-2836(62)80094-1 10.1038/2051171a0 |
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| SubjectTerms | Amino Acids - analysis Antigens - classification Bence Jones Protein - classification Carboxypeptidases Chromatography, Gel Humans Immunoglobulin G - classification Multiple Myeloma - immunology Serine |
| Title | Use of carboxypeptidase A for simultaneous assessment of purity and assignment of human Bence Jones proteins and light chains to K and L classes |
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