In vivo augmentation of IFN-γ with a rIL-12 human/mouse chimera: Pleiotropic effects against infectious agents in mice and rats

• Published in: Cytokine (Philadelphia, Pa.) Vol. 6; no. 3; pp. 318 - 328
• Main Authors: Gladue, Ronald P., Laquerre, Ann M., Magna, Holly A., Carroll, Laurie A., O'Donnell, Michele, Changelian, Paul S., Franke, Arthur E.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.1994
• Subjects: Animals; Antibodies - pharmacology; Cell Line; E. coli; Escherichia coli Infections - immunology; Female; Growth Substances - pharmacology; Growth Substances - therapeutic use; Humans; IFN-γ; IL-12; Interferon-gamma - biosynthesis; Interferon-gamma - blood; Interleukin-12; Interleukins - biosynthesis; Interleukins - pharmacology; Interleukins - therapeutic use; L. monocytogenes; Lipopolysaccharides - toxicity; Listeria monocytogenes - drug effects; Listeria monocytogenes - growth & development; Listeriosis - immunology; Listeriosis - therapy; Liver - drug effects; Liver - microbiology; Lung - drug effects; Lung - microbiology; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Rats; Recombinant Fusion Proteins - biosynthesis; Recombinant Fusion Proteins - pharmacology; Recombinant Fusion Proteins - therapeutic use; Staphylococcal Infections - immunology; Staphylococcal Infections - therapy; Transfection; Tumor Cells, Cultured; Tumor Necrosis Factor-alpha - biosynthesis; Virus; Virus Diseases - immunology; Virus Diseases - therapy; Virus; IFN-γ; L. monocytogenes; IL-12; E. coli

A heterodimer containing the mouse 35 kDa and human 40 kDa subunit of IL-12 was expressed in COS cells (cIL-12). Administration of 25–200 U of the cIL-12-COS supernatant to mice twice daily for 2 days augmented spleen cell IFN-γ production in response to IL-2 and peritoneal macrophage activity (supe...