Increased hydroxyl radical production in liver peroxisomal fractions from rats treated with peroxisome proliferators

• Published in: Carcinogenesis (New York) Vol. 7; no. 5; p. 795
• Main Authors: Elliott, B M, Dodd, N J, Elcombe, C R
• Format: Journal Article
• Language: English
• Published: England 01.05.1986
• Subjects: Animals; Azides - toxicity; Cell Division - drug effects; Cyanides - toxicity; Cyclic N-Oxides; DNA; Electron Spin Resonance Spectroscopy; Hydrogen Peroxide - toxicity; Hydroxides - metabolism; Hydroxyl Radical; In Vitro Techniques; Liver - drug effects; Liver - metabolism; Male; Microbodies - drug effects; Microbodies - metabolism; Pentetic Acid - pharmacology; Rats; Rats, Inbred Strains

Electron spin resonance (e.s.r.), using the spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), has been employed to measure hydroxyl radical production in liver peroxisome-enriched fractions isolated from male Alpk/Ap rats administered chemicals known to cause peroxisome proliferation. The DMPO-OH a...