Effect of anions on the structure of glyceraldehyde-3-phosphate dehydrogenase from rat skeletal muscle

• Published in: Biochemical and biophysical research communications Vol. 43; no. 4; pp. 840 - 846
• Main Authors: Nagradova, N.K., Guseva, M.K.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 21.05.1971
• Subjects: Acetates; Animals; Bromides; Chemical Phenomena; Chemistry; Chlorides; Citrates; Dialysis; Drug Stability; Glyceraldehyde-3-Phosphate Dehydrogenases - antagonists & inhibitors; Ions; Muscles - enzymology; Nitrates; Phosphates; Potassium; Potassium Chloride; Potassium Iodide; Quaternary Ammonium Compounds; Rats; Sodium; Sodium Chloride; Sulfates; Temperature; Ultracentrifugation

Glyceraldehyde-3-phosphate dehydrogenase from rat skeletal muscle undergoes reversible partial dissociation into 4.2–4.5 S fragments at +4°C and at protein concentrations as high as 8–9 mg/ml, depending upon the anion species present. The order of effectiveness of various anions in dissociating acti...