Effect of Species, Fertilization and Harvest Date on Microbial Composition and Mycotoxin Content in Forage

The aim of the project was to evaluate the potential of microbial threat to feed safety in the year 2018. Analyses of the epiphytic community of several forage species (clovers, cocksfoot, fescue, festulolium, perennial ryegrass, timothy and trefoil) in variants of fertilized and non-fertilized vege...

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Published inAgriculture (Basel) Vol. 9; no. 5; p. 102
Main Authors Baholet, Daria, Kolackova, Ivana, Kalhotka, Libor, Skladanka, Jiri, Haninec, Peter
Format Journal Article
LanguageEnglish
Published MDPI AG 01.05.2019
Subjects
clover
deoxynivalenol
epiphytic microflora
fungi
grass
zearalenone
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Abstract The aim of the project was to evaluate the potential of microbial threat to feed safety in the year 2018. Analyses of the epiphytic community of several forage species (clovers, cocksfoot, fescue, festulolium, perennial ryegrass, timothy and trefoil) in variants of fertilized and non-fertilized vegetation were performed. The hypothesis is based on the fact that microorganisms are normally present on plant material during its growth all the way from the seed to the senescence; they are influenced by a plant’s fitness, and they affect its harvest and utilization. Microflora was analyzed by cultivation on specific substrates, total microbial count and five specific microbial groups were observed and quantified. Forage species did not affect plant microflora. The highest risk factor of microbial contamination of feed was proved to be harvest date. Mycotoxin contamination of fresh feed was determined (deoxynivalenol and zearalenone) using ELISA. Zearalenone (ZEA) levels were negatively correlated to fertilization intensity, although these results were not statistically significant. Deoxynivalenol (DON) levels were the lowest in a moderate fertilization regime. Significant differences in mycotoxin content were found among botanical species.
AbstractList The aim of the project was to evaluate the potential of microbial threat to feed safety in the year 2018. Analyses of the epiphytic community of several forage species (clovers, cocksfoot, fescue, festulolium, perennial ryegrass, timothy and trefoil) in variants of fertilized and non-fertilized vegetation were performed. The hypothesis is based on the fact that microorganisms are normally present on plant material during its growth all the way from the seed to the senescence; they are influenced by a plant’s fitness, and they affect its harvest and utilization. Microflora was analyzed by cultivation on specific substrates, total microbial count and five specific microbial groups were observed and quantified. Forage species did not affect plant microflora. The highest risk factor of microbial contamination of feed was proved to be harvest date. Mycotoxin contamination of fresh feed was determined (deoxynivalenol and zearalenone) using ELISA. Zearalenone (ZEA) levels were negatively correlated to fertilization intensity, although these results were not statistically significant. Deoxynivalenol (DON) levels were the lowest in a moderate fertilization regime. Significant differences in mycotoxin content were found among botanical species.
Author Skladanka, Jiri
Baholet, Daria
Kalhotka, Libor
Kolackova, Ivana
Haninec, Peter
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CitedBy_id crossref_primary_10_1371_journal_pone_0288397
crossref_primary_10_3390_agriculture12020133
crossref_primary_10_3390_toxins13070460
crossref_primary_10_3390_toxins12100648
crossref_primary_10_3390_microorganisms10061154
crossref_primary_10_3390_agriculture12020204
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SubjectTerms clover
deoxynivalenol
epiphytic microflora
fungi
grass
zearalenone
Title Effect of Species, Fertilization and Harvest Date on Microbial Composition and Mycotoxin Content in Forage
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