Fibrin polymerization rate at different temperatures and in the presence of fragment D

• Published in: Thrombosis research Vol. 21; no. 3; pp. 237 - 245
• Main Authors: Belitser, V.A., Varetskaja, T.V., Musjalkovakaja, A.A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01.02.1981
• Subjects: Animals; Cattle; clotting time; Fibrin; Fibrin Fibrinogen Degradation Products - pharmacology; Fibrin polymerization rate; Light; light scattering; Polymers; Scattering, Radiation; Temperature; Whole Blood Coagulation Time; Fibrin polymerization rate; clotting time; light scattering
• ISSN: 0049-3848; 1879-2472
• DOI: 10.1016/0049-3848(81)90161-4

Temperature dependence of fibrin monomer polymerisation has been examined by registration of: I) light scattering, 2) clotting time and 3) clotted protein accumulation. The ratio of rates at 35 and 15°C is found to be about 3.5. The reduction in polymerization-caused turbidity due to the presence of fragment D is shown to result mainly from the increased transparency of the appearing polymers of fibrin. In systems containing fragment D, polymerization kinetics can be studied by the clotting-time method. The optical method is not applicable (unless fragment D ooncentration is very low) as no definite maximum of light scattering is reached making curve normalization impossible. Thus, the still accepted views that the antiolotting effect of fibrin (ogen) fragments is measurable directly by light scattering changes and that fibrin monomer polymerization is vertually temperature-independent have been revised.