The effect of glucocorticoids on adipocyte corticotropin receptors and adipocyte responses

• Published in: Biochimica et biophysica acta Vol. 672; no. 3; pp. 268 - 279
• Main Authors: Behrens, Catherine M., Ramachandran, J.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.02.1981
• Subjects: Adipocyte; Adipose Tissue - drug effects; Adipose Tissue - metabolism; Adrenalectomy; Adrenocorticotropic Hormone - metabolism; Adrenocorticotropic Hormone - pharmacology; Animals; Cell Count; Corticotropin receptor; Cyclic AMP - metabolism; Dexamethasone - pharmacology; DNA - analysis; Epinephrine - pharmacology; Glucocorticoid regulation; Hormone binding; Male; Rats; Receptors, Cell Surface - drug effects; Receptors, Corticotropin; ACTH; Adipocyte; adrenocorticotropin; Glucocorticoid regulation; Hormone binding; Corticotropin receptor

A specific and sensitive assay for determining the binding of adrenocorticotropin (ACTH) to isolated rat adipocytes has been developed and utilized to study the effect of glucocorticoids on ACTH receptor. Measurement of the binding of tritiated ACTH (spec. act. 90 Ci/mmol) to adipocytes isolated fro...