An automated fluorometric procedure for histidine in blood

• Published in: Clinica chimica acta Vol. 36; no. 1; pp. 105 - 111
• Main Authors: Hill, Hoyle D., Summer, George K., Newton, Dale A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 1972
• Subjects: Administration, Oral; Aldehydes; Amino Acid Metabolism, Inborn Errors - blood; Amino Acid Metabolism, Inborn Errors - diagnosis; Autoanalysis; Chemistry, Clinical - instrumentation; Fluorometry; Histidine - administration & dosage; Histidine - blood; Humans; Infant, Newborn; Kinetics; Methods; Phthalic Acids

We have adapted the sample procurement procedure of blood spotted on filter paper for use with an automated fluorometric method for histidine which is based on the reaction of the amino acid with O-phthaldialdehyde in alkaline solution. Histidine was eluted from blood spotted on filter paper by an a...