Impact of variation in reagent combinations for one‐stage clotting assay on assay discrepancy in nonsevere haemophilia A

• Published in: International journal of laboratory hematology Vol. 43; no. 1; pp. 131 - 138
• Main Authors: Suzuki, Atsuo, Suzuki, Nobuaki, Kanematsu, Takeshi, Okamoto, Shuichi, Tamura, Shogo, Kikuchi, Ryosuke, Katsumi, Akira, Kiyoi, Hitoshi, Kojima, Tetsuhito, Matsushita, Tadashi
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.02.2021
• Subjects: Amino Acid Substitution; assay discrepancy; Biological Assay; Blood Coagulation Tests; chromogenic substrate assay; Clotting; Coagulation factors; factor VIII; Factor VIII - genetics; Factor VIII - metabolism; Factor VIII deficiency; Genotypes; haemophilia A; Hemophilia; Hemophilia A - blood; Hemophilia A - genetics; Humans; Indicators and Reagents; Male; Mutation, Missense; one‐stage clotting assay; Reagents; Variation; haemophilia A; assay discrepancy; one-stage clotting assay; factor VIII; chromogenic substrate assay

Introduction Factor VIII activity (FVIII:C) is measured by one‐stage clotting assay (OSA) or chromogenic substrate assay (CSA). Significant differences in FVIII:C between OSA (FVIII:C1st) and CSA (FVIII:CChr) are described as assay discrepancy in nonsevere haemophilia A (HA). A large number of reage...