Spatial Positioning and Matrix Programs of Cancer-Associated Fibroblasts Promote T-cell Exclusion in Human Lung Tumors

• Published in: Cancer discovery Vol. 12; no. 11; pp. 2606 - 2625
• Main Authors: Grout, John A., Sirven, Philemon, Leader, Andrew M., Maskey, Shrisha, Hector, Eglantine, Puisieux, Isabelle, Steffan, Fiona, Cheng, Evan, Tung, Navpreet, Maurin, Mathieu, Vaineau, Romain, Karpf, Lea, Plaud, Martin, Begue, Anne-Laure, Ganesh, Koushik, Mesple, Jérémy, Casanova-Acebes, Maria, Tabachnikova, Alexandra, Keerthivasan, Shilpa, Lansky, Alona, Berichel, Jessica Le, Walker, Laura, Rahman, Adeeb H., Gnjatic, Sacha, Girard, Nicolas, Lefevre, Marine, Damotte, Diane, Adam, Julien, Martin, Jerome C., Wolf, Andrea, Flores, Raja M., Beasley, Mary Beth, Pradhan, Rachana, Muller, Soren, Marron, Thomas U., Turley, Shannon J., Merad, Miriam, Kenigsberg, Ephraim, Salmon, Hélène
• Format: Journal Article
• Language: English
• Published: United States 02.11.2022
• Subjects: Cancer-Associated Fibroblasts - pathology; Fibroblasts; Humans; Immunotherapy - methods; Life Sciences; Lung Neoplasms - pathology; T-Lymphocytes; Tumor Microenvironment

It is currently accepted that cancer-associated fibroblasts (CAF) participate in T-cell exclusion from tumor nests. To unbiasedly test this, we used single-cell RNA sequencing coupled with multiplex imaging on a large cohort of lung tumors. We identified four main CAF populations, two of which are a...