Twist is required for muscle development of the adult legs in Henosepilachna vigintioctopunctata

• Published in: Archives of insect biochemistry and physiology Vol. 115; no. 1; pp. e22063 - n/a
• Main Authors: Anjum, Ahmad Ali, Lin, Meng‐Jiao, Jin, Lin, Li, Guo‐Qing
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.01.2024
• Subjects: Adults; Animals; Coleoptera - genetics; Copulation; Developmental stages; Drosophila melanogaster; Foliage; Gene expression; Henosepilachna vigintioctopunctata; Insects; Larva - genetics; Leg; Legs; Muscle Development; Muscles; Myogenesis; Ovaries; Ovarioles; Oviposition; Pupation; RNA Interference; RNA-mediated interference; RNAi; Tibia; Twist; Vitellogenesis; wing; Twist; RNAi; wing; Henosepilachna vigintioctopunctata; leg
• ISSN: 0739-4462; 1520-6327
• DOI: 10.1002/arch.22063

Although muscle development has been widely studied in Drosophila melanogaster, it was a great challenge to apply to developmental processes of other insect muscles. This study was focused on the functional characterization of a basic helix‐loop‐helix transcription factor gene twist in an herbivorous ladybird Henosepilachna vigintioctopunctata. Its transcript (Hvtwist) levels were detected in all developmental stages. RNA interference (RNAi)‐aided knockdown of Hvtwist at the penultimate larval instar stage impaired pupation, and caused a deformed adult in the legs. The tarsi were malformed and did not support the bodies in an upright position. The climbing ability was impaired. Moreover, around 50% of the impaired adults had a malformed elytrum. In addition, they consumed less foliage and did not lay eggs. A hematoxylin–eosin staining of the leg demonstrated that the tibial extensor (TE) and the tibial flexor (TF) muscles were originated from the femurs while levator and depressor muscles of the tarsus (TL and TD) were located in the tibia in the control adults, in which tarsal segments were devoid of muscles. RNAi treatment specific to Hvtwist expression markedly impaired TE and TF muscles in the femurs, and prevented the development of TL and TD muscles in the tibia. Therefore, our findings demonstrate Twist plays a vital role in the myogenesis in H. vigintioctopunctata adult legs. RNA interference (RNAi) of Hvtwist inhibits reduced pupation and caused deformed adults. All the Hvtwist RNAi adults possessed deformed legs and undeveloped muscles. The climbing ability of the Hvtwist RNAi adults was impaired. Highlights Previtellogenesis occurs in 4‐day‐old H. vigintioctopunctata ovarioles. The vitellogenesis happens in the 5–7‐day‐old ovaries. The choriogenesis take places at 8 and 9 days posteclosion. The copulation and oviposition comes about from 8 days postemergence.