Study of the mass spectrometric fragmentation of pseudouridine: comparison of fragmentation data obtained by matrix-assisted laser desorption/ionisation post-source decay, electrospray ion trap multistage mass spectrometry, and by a method utilising electrospray quadrupole time-of-flight tandem mass spectrometry and in-source fragmentation

• Published in: Rapid communications in mass spectrometry Vol. 19; no. 21; pp. 3075 - 3085
• Main Authors: Dudley, Edward, Tuytten, Robin, Bond, Alison, Lemière, Filip, Brenton, A. Gareth, Esmans, Eddy L., Newton, Russell P.
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.01.2005
• Subjects: Pseudouridine - analysis; Pseudouridine - chemistry; Spectrometry, Mass, Electrospray Ionization - instrumentation; Spectrometry, Mass, Electrospray Ionization - methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods

Many nucleosides and their modified forms have been studied by mass spectrometry elaborating the detailed fragmentation pathways under MS2 and MSn conditions. Although the C‐nucleoside pseudouridine has been fragmented and studied briefly, usually amongst many other nucleosides, it has not been inve...