Development and validation of a rapid and sensitive UPLC–MS/MS method for quantification of kukoamine B in human plasma: Application to a clinical pharmacokinetic study

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 132; pp. 1 - 6
• Main Authors: Wang, Zhenlei, Zhao, Qian, Li, Lili, Hu, Pei, Dong, Kai, Chen, Shuai, Jiang, Ji
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05.01.2017
• Subjects: Caffeic Acids - blood; Caffeic Acids - pharmacokinetics; Calibration; China; Chromatography, High Pressure Liquid - methods; Drug Stability; Healthy Volunteers; Human plasma; Humans; Kukoamine B; Linear Models; Pharmacokinetic; Quality Control; Reproducibility of Results; Sensitivity and Specificity; Sepsis; Solid Phase Extraction; Solvents - chemistry; Spermine - analogs & derivatives; Spermine - blood; Spermine - pharmacokinetics; Tandem Mass Spectrometry - methods; Time Factors; UPLC–MS/MS; Sepsis; Human plasma; Kukoamine B; UPLC–MS/MS; Pharmacokinetic

[Display omitted] •This is the first study on quantifying kukoamine B in human plasma.•A UPLC–MS/MS method with a short run time of 1.5min was established.•The method was proved to be rapid, accurate and robust.•The method was successfully applied to a pharmacokinetic study of kukoamine B in healthy...