Agitation-Induced Aggregation of Lysine- And Interchain Cysteine-Linked Antibody-Drug Conjugates

• Published in: Journal of pharmaceutical sciences Vol. 113; no. 5; pp. 1265 - 1274
• Main Authors: Johann, Florian, Wöll, Steffen, Winzer, Matthias, Gieseler, Henning
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2024
• Subjects: Antibodies, Monoclonal; Antibody–drug conjugate(s) (ADC); Conjugation; Cysteine; Developability; High-throughput technology(s); Hydrophobic and Hydrophilic Interactions; Immunoconjugates; Lysine; Monoclonal antibody(s); Physical stability; Protein aggregation; Protein aggregation; High-throughput technology(s); Developability; Antibody–drug conjugate(s) (ADC); Conjugation; Monoclonal antibody(s); Physical stability

Drug conjugation to an antibody can affect its stability, which depends on factors such as the conjugation technique used, drug-linker properties, and stress encountered. This study focused on the effects of agitation stress on the physical stability of two lysine (ADC-K) and two interchain cysteine...