Differential-display PCR of peripheral blood for biomarker discovery in chronic fatigue syndrome

• Published in: Journal of molecular medicine (Berlin, Germany) Vol. 82; no. 11; pp. 750 - 755
• Main Authors: Steinau, Martin, Unger, Elizabeth R., Vernon, Suzanne D., Jones, James F., Rajeevan, Mangalathu S.
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.11.2004; Springer Nature B.V
• Subjects: Biological and medical sciences; Chronic fatigue syndrome; Fatigue Syndrome, Chronic - epidemiology; Fatigue Syndrome, Chronic - genetics; Fatigue Syndrome, Chronic - physiopathology; Female; Gene Expression Profiling; General aspects; Genetic Markers; Genetic Predisposition to Disease; Humans; Male; Medical sciences; Prevalence; Real-time reverse-transcription PCR; Gene expression profiling; Biological marker; Biological indicator; Discoveries; Real time; Chronic fatigue syndrome; Blood; Gene expression profile; Differential display PCR; Medicine; Polymerase chain reaction; Biomarkers; Molecular biology; Reverse transcription polymerase chain reaction

We used differential-display PCR of peripheral blood mononuclear cells (PBMCs) to search for candidate biomarkers for chronic fatigue syndrome (CFS). PBMCs were collected from a subject with CFS and an age- and sex-matched control before and 24 h after exercise. RNA expression profiles were generate...