Upregulation of miR-98 Inhibits Apoptosis in Cartilage Cells in Osteoarthritis
We aimed to investigate the effects of microRNA-98 (miR-98) on apoptosis in cartilage cells of osteoarthritis (OA) patients. Knee cartilage tissue samples were collected from 31 OA patients, 21 autopsies, and 26 amputation patients due to trauma. The clinicopathological data were recorded. Quantitat...
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Published in | Genetic testing and molecular biomarkers Vol. 20; no. 11; p. 645 |
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Format | Journal Article |
Language | English |
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United States
01.11.2016
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Abstract | We aimed to investigate the effects of microRNA-98 (miR-98) on apoptosis in cartilage cells of osteoarthritis (OA) patients.
Knee cartilage tissue samples were collected from 31 OA patients, 21 autopsies, and 26 amputation patients due to trauma. The clinicopathological data were recorded. Quantitative real-time polymerase chain reaction was performed to compare the miR-98 expression levels from cartilage cells obtained from the OA and non-OA patients. Clinicopathological characteristics of the patients were also analyzed. Primary chondrocytes were separated from cartilage tissues and transfected with plasmids or siRNA to overexpress or inhibit miR-98. Annexin V-PI double staining and TUNEL assays were used to examine apoptosis in the primary chondrocytes after transfection. Finally, a rat OA model was used to confirm the effects of miR-98 on apoptosis in cartilage cells in vivo.
Compared with the normal cartilage tissues, miR-98 expression was reduced in the OA cartilage tissues (p < 0.01). The miR-98 expression levels were also significantly correlated with the OA stage (p < 0.05). In vitro, transfection with the miR-98 inhibitor increased apoptosis in the cartilage cells (p < 0.05), and transfection with a miR-98 mimic inhibited apoptosis in cartilage cells (p < 0.05). In the OA rat model, exogenous injection of the miR-98 mimic inhibited apoptosis in the rat cartilage cells thus alleviating OA.
MiR-98 expression is reduced in the cartilage cells of OA patients and the overexpression of miR-98 inhibits cartilage cell apoptosis, while inhibition of microRNA-98 leads to cartilage cell apoptosis. These findings provide a theoretical basis for the development of novel targeted therapies for OA. |
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AbstractList | We aimed to investigate the effects of microRNA-98 (miR-98) on apoptosis in cartilage cells of osteoarthritis (OA) patients.
Knee cartilage tissue samples were collected from 31 OA patients, 21 autopsies, and 26 amputation patients due to trauma. The clinicopathological data were recorded. Quantitative real-time polymerase chain reaction was performed to compare the miR-98 expression levels from cartilage cells obtained from the OA and non-OA patients. Clinicopathological characteristics of the patients were also analyzed. Primary chondrocytes were separated from cartilage tissues and transfected with plasmids or siRNA to overexpress or inhibit miR-98. Annexin V-PI double staining and TUNEL assays were used to examine apoptosis in the primary chondrocytes after transfection. Finally, a rat OA model was used to confirm the effects of miR-98 on apoptosis in cartilage cells in vivo.
Compared with the normal cartilage tissues, miR-98 expression was reduced in the OA cartilage tissues (p < 0.01). The miR-98 expression levels were also significantly correlated with the OA stage (p < 0.05). In vitro, transfection with the miR-98 inhibitor increased apoptosis in the cartilage cells (p < 0.05), and transfection with a miR-98 mimic inhibited apoptosis in cartilage cells (p < 0.05). In the OA rat model, exogenous injection of the miR-98 mimic inhibited apoptosis in the rat cartilage cells thus alleviating OA.
MiR-98 expression is reduced in the cartilage cells of OA patients and the overexpression of miR-98 inhibits cartilage cell apoptosis, while inhibition of microRNA-98 leads to cartilage cell apoptosis. These findings provide a theoretical basis for the development of novel targeted therapies for OA. |
Author | Wu, Yu-Bo Li, Cui-Yun Liu, Jia-Tian Wang, Gui-Long |
Author_xml | – sequence: 1 givenname: Gui-Long surname: Wang fullname: Wang, Gui-Long organization: 1 Department of Orthopedics, Linyi People's Hospital , Linyi, P.R. China – sequence: 2 givenname: Yu-Bo surname: Wu fullname: Wu, Yu-Bo organization: 1 Department of Orthopedics, Linyi People's Hospital , Linyi, P.R. China – sequence: 3 givenname: Jia-Tian surname: Liu fullname: Liu, Jia-Tian organization: 1 Department of Orthopedics, Linyi People's Hospital , Linyi, P.R. China – sequence: 4 givenname: Cui-Yun surname: Li fullname: Li, Cui-Yun organization: 2 Department of Pathology, Linyi People's Hospital , Linyi, P.R. China |
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Keywords | cartilage cells TUNEL assay osteoarthritis Annexin V-PI double staining assay apoptosis siRNA miR-98 |
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Snippet | We aimed to investigate the effects of microRNA-98 (miR-98) on apoptosis in cartilage cells of osteoarthritis (OA) patients.
Knee cartilage tissue samples were... |
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SubjectTerms | Adult Aged Animals Apoptosis - genetics Cartilage - metabolism Cartilage - pathology Chondrocytes - metabolism Chondrocytes - pathology Disease Models, Animal Female Humans Male MicroRNAs - genetics MicroRNAs - metabolism Middle Aged Osteoarthritis, Knee - genetics Osteoarthritis, Knee - metabolism Osteoarthritis, Knee - pathology Rats Rats, Sprague-Dawley RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Small Interfering - genetics Up-Regulation |
Title | Upregulation of miR-98 Inhibits Apoptosis in Cartilage Cells in Osteoarthritis |
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