Microparticles of Aloe vera/vitamin E/chitosan: Microscopic, a nuclear imaging and an in vivo test analysis for burn treatment

• Published in: European journal of pharmaceutics and biopharmaceutics Vol. 86; no. 2; pp. 292 - 300
• Main Authors: Pereira, Gabriela Garrastazu, Santos-Oliveira, Ralph, Albernaz, Martha S., Canema, Daniel, Weismüller, Gilberto, Barros, Eduardo Bede, Magalhães, Luciana, Lima-Ribeiro, Maria Helena Madruga, Pohlmann, Adriana Raffin, Guterres, Silvia S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2014
• Subjects: Aloe - chemistry; Animals; Atomic force microscopy; Burn; Burns - drug therapy; Chemistry, Pharmaceutical - methods; Chitosan - administration & dosage; Chitosan - chemistry; Drug Delivery Systems - methods; Female; Gels - administration & dosage; Gels - chemistry; In vivo cicatrization; Male; Mice; Microspheres; Nanoparticles - administration & dosage; Nanoparticles - chemistry; Particle Size; Skin; Skin - metabolism; Technetium-99m; Tissue Distribution; Vitamin E - administration & dosage; Vitamin E - chemistry; Wound Healing - drug effects; Burn; Atomic force microscopy; Skin; Technetium-99m; In vivo cicatrization
• ISSN: 0939-6411; 1873-3441
• DOI: 10.1016/j.ejpb.2013.10.011

The use of drug-loaded nanoparticles and microparticles has been increasing, especially for cosmetic and drug delivery purposes. In this work, a new microparticle formulation was developed for use in the healing process of skin burns in a composition of Aloe vera/vitamin E/chitosan. In order to observe the morphological properties, Raman and atomic force microscopy evaluation were performed. The biodistribution studies were analyzed by using a nuclear methodology, labeling the microparticles with Technetium-99m and in vivo test was procedure to analyzed the cicatrization process. The results of AFM analysis show the formation and the adherence property of the microparticles. Raman analyses show the distribution of each component in the microparticle. The nuclear method used shows that the biodistribution of the microparticles remained in the skin. The in vivo cicatrization test showed that the poloxamer gel containing the microparticles make a better cicatrization in relation to the other formulations tested.