Demonstration that endoplasmic reticulum-associated degradation of glycoproteins can occur downstream of processing by endomannosidase

• Published in: Biochemical journal Vol. 438; no. 1; p. 133
• Main Authors: Kukushkin, Nikolay V, Alonzi, Dominic S, Dwek, Raymond A, Butters, Terry D
• Format: Journal Article
• Language: English
• Published: England 15.08.2011
• Subjects: Animals; Blotting, Western; CHO Cells; Cricetinae; Cricetulus; Endoplasmic Reticulum - metabolism; Fluorescent Antibody Technique; Glycoproteins - metabolism; Glycosylation; Golgi Apparatus - metabolism; Humans; Mannans; Mannosidases - genetics; Mannosidases - metabolism; Oligosaccharides - metabolism; Protein Processing, Post-Translational; Protein Transport

During quality control in the ER (endoplasmic reticulum), nascent glycoproteins are deglucosylated by ER glucosidases I and II. In the post-ER compartments, glycoprotein endo-α-mannosidase provides an alternative route for deglucosylation. Previous evidence suggests that endomannosidase non-selectiv...