Specific binding reactions monitored with ligand-cofactor conjugates and bacterial luciferase

• Published in: Analytical biochemistry Vol. 72; no. 1; pp. 283 - 292
• Main Authors: Schroeder, Hartmut R., Carrico, Robert J., Boguslaski, Robert C., Christner, James E.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 07.05.1976
• Subjects: Alcohol Oxidoreductases - metabolism; Antigen-Antibody Reactions; Avidin - pharmacology; Binding Sites; Biotin; Dinitrofluorobenzene; Flavin Mononucleotide; Ligands; Luciferases; Methods; NAD - analogs & derivatives; Protein Binding

A unique rapid method for assaying specific binding reactions with ligand-cofactor conjugates and a bioluminescent reaction is described. Biotin and 2,4-dinitrofluorobenzene were coupled covalently to the free amino residue of nicotinamide 6-(2-aminoethylamino) purine dinucleotide (AENAD) to produce...